1.
Response Of Cryopreserved Nili Ravi Buffalo Bull Semen To Gallic Acid Inclusion In Semen Extender
by Muhammad Tariq | Dr. Imtiaz Rabbani | Dr. Hafsa Zaneb | Dr. Muhammad Shahbaz Yousaf | Faculty of Biosciences.
Material type: ; Format:
print
Publisher: 2012Dissertation note: The major population of buffalo in the world (more than 75%) is located in Asia and 14% in Pakistan, where buffaloes play an important role in rural livestock production providing milk, meat and work draft force. Genetically the ratio of proven buffalo bulls is lesser than cattle bulls, and additionally the volume of semen and spermatozoa concentration is also lower than cattle semen. The success of inseminating buffalos with cryopreserved semen is also low, which account for major cause of fertility problems.
During cryopreservation, the spermatozoa faces osmochemical, mechanical and thermal stresses, which are predominant at dilution, cooling, equilibration, freezing and thawing stages which lead to damage spermatozoa functional and morphological characteristics. Beside these exogenous stresses, oxidative stress damages the spermatozoa endogenously. Keeping in mind all these problems, the reduction of these stresses through inclusion of antioxidants in semen may improve its quality and ultimately the fertility of buffalo bulls. This may be obtained from antioxidant addition to extender at the time of cryopreservation. Gallic acid possess good antioxidative properties, the use of this polyphenolic compound may reduce oxidation in buffalo bull semen.
In the current study, semen from four (n=4) healthy Nili Ravi buffalo bulls was collected by artificial vagina and GA was added to the semen @ 1 µM, 15 µM, 30 µM, 45 µM, 60 µM, and 100 µM and a total of six groups were prepared. One group was kept control and no GA was added to that group. The routine quality evaluation of semen for motility and concentration was made, extender was added, then semen cooled to 4°C filled in 0.5mL straws for 4 hours and frozen in liquid nitrogen at -196 °C.
The semen was then transported to the Physiology Laboratory of UVAS for further evaluations. The parameters evaluated were percentage motility, plasma membrane integrity (HOST assay), acrosomal integrity (NAR), viability (Live/Dead), DNA integrity (Acridine orange assay) and oxidative stress (TBARS assay). Five straws from each GA group were thawed individually in water bath at 37°C for 30 seconds and evaluated for quality parameters. The data collected was presented as cells ± SE and treatment groups were compared using one way analysis of variance. The group differences were compared by using the Duncan Multiple Range Test. The results revealed that GA improved (P<0.05) the spermatozoa viability and plasma membrane integrity.
In conclusion, the addition of 15 µM GA to semen extender improved marginally the buffalo bull spermatozoa motility, viability and membrane integrity but still not sufficient to reach the statistical significance, while it has no protective effects on other parameters like Acrosomal integrity, DNA status and oxidative stress. However further studies are needed to assess the role of GA in different concentrations and other animals.
Availability: Items available for loan: UVAS Library [Call number: 1456,T] (1).
2.
Effect Of Lipoic Axid Inclusion In Semen Extender On The Cryopreservation Of Sahiwal Cattle Semen
by Sheeraz Ghani | Dr. Muhammad Shahbaz Yousaf | Dr. Hafsa Zaneb | Dr. Imtiaz Rabbani.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1725,T] (1).
3.
Single Or Combined Effects Of Probiotics And Gallic Acid Supplementation On Serum Biochemistry In Dexamethasone
by Fahad mahmood | Dr. Muhammad shahbaz yousaf | Dr. Hafsa zaneb | Prof. Dr. habib ur rehman.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2045,T] (1).
4.
Response Of Lactobacillus Based Probiotics Supplementation On Zootechnical Parameters Bone Health
by Hira Najeeb | Dr. Muhammad Shahbaz Yousaf | Dr. Hafsa Zaneb | Prof. Dr. Habib ur Rehman.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2099,T] (1).
5.
Single And Combned Effect Of Turmeric And Probiotic Supplementation On Serum Lioid And Creatinine Profile In Alloxan Induced Diabetic Rats
by Salman Zafar | Dr. Muhammad Shahbaz Yousaf | Dr. Hafsa Zaneb | Prof. Dr. Habib ur Rehman.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2106,T] (1).
6.
In Vitro Effects Of Bisphenol-A On The Electrophysiology Of Isolated Jejunum Of Layers
by Nabeela Hussain (2013-VA-863) | Dr Imtiaz Rabbani | Dr. Muhammad Shahbaz Yousaf | Dr. Hafsa Zaneb.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: A major nutritional problem these days is the biological and chemical contamination of food with environmental chemicals; Bisphenol A is one of these chemicals which cause contamination of food. BPA have many genomic effects, it is consider as endocrine disrupting hormone. However BPA have many non genomic effects e.g BPA enter into the body orally by use of plastic food cans lead through the intestine and liver and affects target organ. These chemicals have also shown adverse effects on development and function of the reproductive organs of the birds (Berg 2000).
Bisphenol A may affect the barrier function of small intestine of layers. Our study aimed to find out the effect of Bisphenol A on the electrophysiology of jejunum of small intestine of layers. For this purpose a total of forty layer birds were purchased from poultry farm. The birds were acclimatized for one week. Following acclimatization one bird was slaughtered daily and jejunum were collected. After stripping the jejunum was divided into four segments and was mounted in four Ussing chambers receiving four treatments as (BPA control, BPA 10 μM, BPA 50 μM and BPA 100 μM) The tissues were first incubate under open circuit conditions for 15 min and then were short circuited by fixing the voltage at 0 mV throughout the experimental period. After 5 minutes following short circuiting the tissues BPA at the concentration of 10 μM, 50 μM and 100 μM, were added on the mucosal side respectively followed by an equilibrium period of ten minutes. The tissue was further monitored for 30 minutes to note any change in electrophysiology is caused by BPA. After this 300uL of concentrated glucose solution was prepared and added to the mucosal side of each chamber to achieve the final concentration of 20mmol/L. The electrical response to glucose addition was observed. ANOVA was used to
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investigate the effect of Bisphenol A on the electrophysiology of jejunum. Followed by Tukey. The P ˂ 0.05 was considered significant.
The results of this study showed that BPA 10μM affects the electrophysiology of intestine as compared to other groups (50μM, 100μM). Suggesting a possible role of BPA in affecting intestinal barrier function and glucose uptake. Availability: Items available for loan: UVAS Library [Call number: 2438-T] (1).
7.
Effects Of Dietary Supplementation Of Thyroxine On Cellular And Humoral Immunity In Broilers
by Muhammad Saad Khilji (2013-VA-594) | Dr. Muhammad Shahbaz Yousaf | Dr. Mansur Abdullah Sandhu | Dr. Muhammad Quaid Zaman | Dr. Hafsa Zaneb.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Over the past few decades broiler industry has showed great improvements in growth, increased efficiency of nutrient utilization. But advances in immune system improvement lag behind significantly. Endocrine system is also a participant in modulating immune response. One of these endocrine systems through which immune system can be modulated is through hypothalamus-pituitary-thyroid axis. Thyroid hormones are known for their role in immune organs development such as thymus and bursa of Fabricius. So this trial was designed to evaluate cell mediated and humoral immune response of birds during hyperthyroidism.
In this trial, one hundred day old broiler chickens were procured and maintained for 14 days. Then birds were divided into three groups. Group 1 received basal diet and served as control. Group 2 and Group 3 served as treatment groups and received 5 μg/Kg and 10 μg/Kg of thyroxine in addition to basal diet for 14 days. After 7, 14 and 21 days of trial, birds were bled and sampling was done. The parameters selected to assess immune response included blood collection for H:L, serum collection, macrophage engulfment performance, NO production assay, delayed type hypersensitivity reaction against phytohemagglutinin, heterophil to lymphocyte ratio, antibody titers against SRBC and viral diseases including ND and IB. Glucose tolerance test was also performed after 7, 14 and 21 days of trial. All the results for the parameters mentioned were analysed statistically using one way ANOVA. Results at p <0.05 were considered significant.
Results revealed showed that hyperthyroidism negatively modulates the immune response of birds. Macrophage phagocytic performance significantly declined, heterophil to
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lymphocyte ratio showed significantly higher. Results for NO production showed increase in control group followed by 10 μg group. Skin response to phytohemagglutinin remained nonsignificant. Antibody titers against SRBC, ND and IBV significantly dropped in birds subjected to hyperthyroidism. Glucose tolerance test showed significantly lower blood glucose levels in treatment groups as compared to treatment groups.
All these results suggest that hyperthyroidism can lead to deficient immune response in challenged birds and that physiological levels of thyroxine are sufficient for maintaining immune system. Availability: Items available for loan: UVAS Library [Call number: 2449-T] (1).
8.
Distribution Of Macro- And Micro-Minerals In Different Tissues Of Broilers Supplemented With Trivalent Chromium
by Abdullah Arif Saeed (2013-VA-862) | Dr. Muhammad Shahbaz Yousaf | Dr. Mansur Abdullah Sandhu | Dr. Muhammad Quaid Zaman | Dr. Hafsa Zaneb.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Broiler chicken is least expensive meat in most of the world’s meat markets. Its demand is getting higher day by day. Broiler producers and researchers are experimenting with variety of supplements to improve feed conversion ratio as well as to make meat healthier and more nutritious. Many mineral supplementations are used commonly in poultry production to attain maximum efficiency. Chromium is one of the micro mineral essential for various body functions. Many research trials had been conducted on trivalent chromium chloride and its other forms observing its effect on carbohydrate, lipid and protein metabolism. No study is yet conducted which is solely based on effect of chromium on other mineral levels in different tissues of body. This study was designed to estimate the effect of chromium chloride supplementation on distribution of macro- and micro-minerals in different tissues of body.
Ninety day old chicks were kept in three groups i.e. two treatment groups and one control group. Treatment groups were divided by the concentration of chromium chloride supplemented in their feed i.e. 12.5 mg Cr/kg feed and 25 mg Cr/kg feed. Sampling was done on 35th day and samples of blood, long bones of hind limb, and thigh muscles were collected. Samples were further processed for estimation of 3 micro and 3 macro minerals. One way ANOVA was used to analyze the data and results were subjected to Tukey’s post-hoc test.
There was no significant effect on muscle and serum mineral content. Calcium seems to decrease in all three bone with supplementation along with potassium. Copper concentration seems to increase in fibula with supplementation.
Availability: Items available for loan: UVAS Library [Call number: 2447-T] (1).
9.
Effects Of Dietary Supplementation Of Moringa Oleifera Leaf Powder On Redox Status, Hepatic And Kidney Function In Rats Exposed To Heat Stress
by Noor Muhammad Khan (2015-VA-07) | Dr. Khalid Abdul Majeed | Dr. Muhammad Shahbaz Yousaf | Dr. Hafsa Zaneb.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: here are a number of stressors which adversely affects the normal homeostasis of the body. One of them is heat stress which has marked damaging effect on the normal physiology of an individual. Moringa oleifera is a versatile plant from Moringaceae family. Moringa oleifera is a best food plant but it also has a large potential as a medicinal plant. Due to rich source of flavonoids, Beta- carotenes, vitamin A & D, Moringa oleifera is known as a best natural anti-oxidant. The leaves of Moringa oleifera also possess hepato-protective and renal protective activities due to its rich anti-oxidant potential.
Hypothesis:It was hypothesized that Moringa oleifera leaf powder supplementation will ameliorate the redox status, hepatic and kidney function in rats exposed to heat stress.
Methodology: Adult male wistar rats (n= 24) were procured and housed in stainless steel cages in the experimental animal shed of department of Physiology, University of Veterinary and Animal Sciences, Lahore-Pakistan. After acclimatization, rats were divided in to four groups (One control and 3 MOLP treated groups). Rats were exposed to high environmental temperature (37±1°C ) for one hour daily(12.00 PM-1.00 PM) throughout the experimental period of 21 days for the induction of heat stress(Sinha. 2007). Anti-stress properties of Moringa oleifera leaf powderwere examined by measuring biochemical parameters, i.e. redox by estimating serum malondialdehyde through assay used by(Ohkawa et al. 1979) and catalase by the same spectrophotometric protocol in accordance with the study (Hadwan et al. 2016). Analysis of liver function enzymes (Aspartate aminotransferase, Alanine aminotransferase and Alkaline phosphatase), kidney function (Blood urea nitrogen, uric acid and serum creatinine), serum
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glucose and lactate dehydrogenasewere carried out by using commercially available kits provided by Human Diagnostics, Germany.
Results:The present study revealed that Moringa oleifera leaf powder supplementation during heat stress has a significant effect (P<0.05) on serum redox status. Serum malondialdehyde and catalase were significantly reduced by Moringa oleifera leaf powder supplementation. On the other hand, Moringa oleifera leaf powder demonstrated a non-significant effect (P>0.05) on hepatic and kidney function. Serum Glucose was also not altered by powdered leaf supplementation of Moringa oleifera.
Statistical analysis: The data was analyzed by using one way analysis of variance (ANOVA) using SPSS software. The group differences were compared by the Tukey’s Test. Differences were considered significant at P<0.05.
Conclusion: From this study, it was concluded that Moringa oleifera leaf powder (MOLP) has a potent antioxidant potential without any marked ameliorative or deteriorative effect on hepatic and kidney functioning in heat stressed rats. Further studies are required to explore the potential of other portions of this plant in heat stress. Availability: Items available for loan: UVAS Library [Call number: 2783-T] (1).
